Descargar Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition (Revised) de PH.D. Behringer, Richard,Transgenic Core and Specialty Resources) Gertsenstein, Marina (Toronto Centre for Phengenomics,Toronto) Nagy, Kristina (Samuel Lunenfeld Research Institute, Mount Sinai Hospital libros ebooks, Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition (Revised) Pdf descargar
Descripci贸n - Cr铆ticas 'The editors...should be praised once again for a brilliant work done, accommodating the latest techniques in mouse embryo manipulation, while not forgetting the traditional procedures that must be learnt properly by anyone joining this field of animal transgenesis for the first time.' ---International Society for Transgenic Technologies Blog Rese帽a del editor This fourth edition of 'The Mouse Manual篓is once again the definitive reference source on mouse development, transgenesis techniques, and molecular biology. Its preeminent authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy have reorganized and updated this edition to include new information and protocols on:路 generation of induced pluripotent stem cells路 RNA microinjections路 lentiviral microinjections and infection路 assisted reproduction techniques for sperm and embryo cryopreservation路 isolation, generation, and transplantation of spermatogonial stem cell lines路 in utero electroporation of gene constructs into postimplantation embryos路 vibratome sectioning of live and fixed tissues for imaging thick tissue sections路 whole-mount fluorescent staining methods for three-dimensional visualizationRecombinant DNA techniques and methods for studies of mouse embryonic development have been updated from previous editions, as has the wealth of information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups. The first edition of this classic work appeared more than 20 years ago, with authors that included Brigid Hogan, Rosa Beddington, Frank Costantini, and Elizabeth Lacy. The field's technological sophistication has grown exponentially but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice.
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